Altogen Biosystems offers comprehensive RNA Transfection Services designed to support cutting-edge research in gene silencing and RNA interference (RNAi). Our services are tailored to assist researchers in the efficient delivery of small interfering RNA (siRNA) molecules into mammalian cells, enabling the precise modulation of gene expression. With expertise in RNA transfection and custom siRNA library development, we provide solutions that enhance the success of gene knockdown experiments and facilitate the discovery of key biological mechanisms. Learn more here.
Overview of RNA Transfection and RNAi
RNA interference (RNAi) is a critical cellular mechanism by which the expression of specific genes is silenced through the introduction of double-stranded RNA (dsRNA). By leveraging synthetic siRNA molecules, typically 21-28 nucleotides in length, researchers can induce RNAi in mammalian cells, effectively reducing gene expression in a sequence-specific manner. This process provides a valuable tool for studying gene functions and elucidating cellular pathways.
However, siRNA transfection is often more challenging than standard DNA or RNA transfections due to the need for special formulations, such as liposomes, to enable the efficient entry of siRNA into cells. Despite these challenges, RNAi offers higher efficiencies compared to stable transfections since siRNA operates in the cytoplasm and does not require entry into the nucleus to trigger gene silencing.
Applications of RNA Transfection
Altogen Biosystems’ RNA transfection services are widely applicable across various research fields, including:
- Gene Function Analysis: By selectively silencing specific genes, researchers can gain insights into their roles in cellular processes and protein expression.
- siRNA Library Development: We offer the development of custom siRNA libraries for high-throughput screening, enabling the identification of genes involved in specific cellular functions or disease phenotypes.
- Drug Development and Off-Target Screening: siRNA libraries can be used to explore potential drug targets and determine off-target effects of gene inhibition technologies such as CRISPR/Cas9.
- RNAi as a Therapeutic Tool: RNAi holds promise as a future therapeutic modality, potentially treating diseases caused by the over-expression of specific proteins, such as certain genetic disorders.
Altogen Biosystems RNA Transfection Services
At Altogen Biosystems, we provide a range of RNAi services to help researchers optimize siRNA transfections and develop tailored siRNA libraries. Our services include, but are not limited to:
- siRNA Transfection Optimization: We work with researchers to fine-tune the conditions for efficient siRNA delivery into various cell types, maximizing gene knockdown efficacy.
- Custom siRNA Design and Synthesis: Our team designs and synthesizes siRNA molecules targeted to your gene of interest, ensuring high specificity and efficiency.
- siRNA Assay Development and Library Screening: Whether your project requires screening a small set of siRNAs or developing an extensive siRNA library, we provide scalable solutions for both low- and high-throughput experiments.
- Gene Knockdown Services: We offer functional analysis of gene knockdown to assess the impact of siRNA-mediated silencing on cellular processes or disease models.